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Measuring herpes simplex virus thymidine kinase reporter gene expression in vitro

✍ Scribed by Yaghoubi, Shahriar S; Gambhir, Sanjiv S


Book ID
109945950
Publisher
Nature Publishing Group
Year
2006
Tongue
English
Weight
215 KB
Volume
1
Category
Article
ISSN
1750-2799

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Two plasmids containing either the complete thymidine kinase gene of Herpes simplex virus type I (pSK2) or the gene without the remote control sequence (pSK1) just behind the lac promoter and the first codons of the lacZ gene were constructed. Both plasmids efficiently transform mouse Ltk- cells as