Multinuclear NMR spectroscopy has been used to study water bound to subtilisin Carlsberg suspended in tetrahydrofuran (THF), with the water itself employed as a probe of the hydration layer's physicochemical and dynamic characteristics. The presence of the enzyme did not affect the intensity, chemic
β¦ LIBER β¦
Measuring enzyme hydration in nonpolar organic solvents using NMR
β Scribed by M. C. Parker; B. D. Moore; A. J. Blacker
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 726 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0006-3592
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