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Measuring Adsorption of a Hydrophobic Probe with a Surface Plasmon Resonance Sensor to Monitor Conformational Changes in Immobilized Proteins

✍ Scribed by Satoshi Yamaguchi; Teruhisa Mannen; Tamotsu Zako; Noriho Kamiya; Teruyuki Nagamune


Book ID
109386779
Publisher
American Institute of Chemical Engineers
Year
2008
Tongue
English
Weight
109 KB
Volume
19
Category
Article
ISSN
8756-7938

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Immobilization of proteins to a carboxym
✍ Bo Johnsson; Stefan LΓΆfΓ₯s; Gabrielle Lindquist πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 1006 KB

A method for fast and simple covalent immobilization of proteins to a carboxymethyldextran-modified gold surface intended for surface plasmon resonance sensors is described. The method utilizes the formation of N-hydroxysuccinimide esters from a fraction of the carboxyl groups of the carboxymethylde