Evaluation of surface hydrophobicity of immobilized protein with a surface plasmon resonance sensor
β Scribed by Satoshi Yamaguchi; Teruhisa Mannen; Teruyuki Nagamune
- Book ID
- 111573594
- Publisher
- Springer Netherlands
- Year
- 2004
- Tongue
- English
- Weight
- 121 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0141-5492
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π SIMILAR VOLUMES
A method for fast and simple covalent immobilization of proteins to a carboxymethyldextran-modified gold surface intended for surface plasmon resonance sensors is described. The method utilizes the formation of N-hydroxysuccinimide esters from a fraction of the carboxyl groups of the carboxymethylde
Behaviors of proteins immobilized on a solid surface were investigated using BIACORE, a biosensor utilizing surface plasmon resonance. This sensor is usually used for analyzing binding events during biomolecular interactions. Here we propose a novel use of this sensor to monitor two kinds of intramo