An interactive computer procedure is described which determines (1)H--(1)H couplings from fitting the cross-peak multiplets in regular phase-sensitive COSY spectra. The robustness and simplicity of the method rely on the fact that a given cross-peak intensity is not an independent variable in the fi
Measurement of vicinal couplings from cross peaks in COSY spectra
β Scribed by Yangmee Kim; J.H Prestegard
- Publisher
- Elsevier Science
- Year
- 1989
- Weight
- 306 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0022-2364
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π SIMILAR VOLUMES
A method in which 1 H-1 H scalar and dipolar couplings are obtained from the cross-peak nulling condition in a series of constanttime (CT) COSY spectra, as a function of the duration of the CT period, is described. The method is best suited for measurement of 1 H-1 H couplings in the range 5-20 Hz.
Two-dimensional pulse sequences for the determination of hetpeak patterns is also available in two-spin systems. No addieronuclear long-range coupling constants are presented. The setional passive spin is required. Purely absorptive phase in quences are based on the HMQC/HMBC or HSQC technique with