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Measurement of serum β2-microglobulin by a latex nephelometric immunoassay

✍ Scribed by F. Iguaz; J. Naval; L. Borque

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
370 KB
Volume
25
Category
Article
ISSN
0009-9120

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✦ Synopsis

A rapid and simple technique for the measurement of beta 2microglobulin on the Behring nephelometer was developed using a commercially available latex-anti [32-microglobulin. This is a fully automated assay and no pretreatment of sample is necessary. An excellent correlation with radioimmunoassay (r = 0.972) and time-resolved fluoroimmunoassay (r = 0.914) was obtained. Haemolysis, lipemia, bilirubin, and rheumatoid factor do not cause interference because of the high dilution of samples and the use of Fab fragments of the antibody. The analytical range extends from 0.6 to 10.7 mg/L. Between-run imprecision (CV) ranged from 6 to 8%.

tured for a turbidimetric system (LA-2000 Eiken Chemical Co Ltd Tokyo, Japan). We used an instrument that is often available in clinical laboratories. Only 40 ~L of reagent/test are required, at a cost of less than $1 per test.

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