Measurement of propericiazine in plasma by capillary gas chromatography and selected ion monitoring detection

✍ Scribed by André P. De Leenheer; Jozef A. Jonckheere; Marc F. Lefevere; Marc E. De Broe; Gert A. Verpooten

• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 435 KB
• Volume: 12
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200120106

✦ Synopsis

A new method, based upon a selective extraction and gas chromatographic/mass spectrometric analysis, was developed to monitor the effect of combined haemoperfusion-haemodialysis treatment in a case of propericiazine poisoning. The method relies on the selected ion monitoring of the acetate derivatives of propericiazine and its internal standard fluphenazine, after their extraction from 1 ml of alkalinized plasma with n-hexane:isopropanol (8:2, v/v), backextraction into an acidified water phase, realkalinization and extraction with n-hexane: isopropanol, derivatization with acetic anhydride and gas chromatography on a short (12 m) OV-101 fused silica capillary column. The described procedure is specific and provides between-assay variability of 4.8% CV at 5 pg I-' plasma concentration. The method enables quantification down to 1 pg I-' and hence demonstrates sufficient sensitivity to permit pharmacokinetic or drug monitoring studies.