Measurement of phenylalanine hydroxylating activity in vivo
β Scribed by C. L. Galli; J. W. Commissiong; F. Karoum; N. H. Neff; E. Costa
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 544 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0364-3190
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π SIMILAR VOLUMES
Hydroxylation of l-phenylalanine (Phe) by hydroxyl radical (\*OH) yields 4-, 3-, and 2-hydroxyl-Phe (para-, meta-, and ortho-tyrosine, respectively). Phe derivative measurements have been employed to detect \*OH formation in cells and tissues, however, the specificity of this assay is limited since
Attack of hydroxyl radical (.OH), generated by a Fenton system at physiological pH, upon L-phenylalanine produces three isomeric tyrosines, o-tyrosine (2-hydroxyphenylalanine), m-tyrosine (3-hydroxyphenylalanine), and p-tyrosine (4-hydroxyphenylalanine). These may be separated by high-performance li