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Measurement of one-bond 15N-13C′ dipolar couplings in medium sized proteins

✍ Scribed by James J. Chou; Frank Delaglio; Ad Bax

Book ID
110262804
Publisher
Springer Netherlands
Year
2000
Tongue
English
Weight
64 KB
Volume
18
Category
Article
ISSN
0925-2738

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✍ Eetu Puttonen; Helena Tossavainen; Perttu Permi 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 304 KB

Four simple and sensitive HNCO-based methods for measurement of 1J(C'Calpha), 1J(NCalpha) and 2J(NCalpha) coupling constants in protein main chains are presented. Three of these experiments enable the simultaneous measurement of 1J(C'Calpha), 1J(NCalpha) and 2J(NCalpha) couplings. Exploitation of th