While the L-arginine conversion assay has been utilized to measure nitric oxide synthase (NOS) activity in isolated enzyme and pure cell preparations, this method often fails to provide accurate measurements in whole tissues. Biological tissues contain variable amounts of unlabeled substrate and enz
Measurement of Nitric Oxide Production in Biological Systems by Using Griess Reaction Assay
β Scribed by Sun, Jie; Zhang, Xueji; Broderick, Mark; Fein, Harry
- Book ID
- 121387214
- Publisher
- Molecular Diversity Preservation International
- Year
- 2003
- Tongue
- English
- Weight
- 155 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1424-8220
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The application and optimization of a microsensor for in situ measurements of nitric oxide (NO) in biological systems are described. The sensor (diameter OS-O.8 pm), exhibiting a response time better than 10 ms and a detection limit of 10 nM, consists of several layers of p-type semiconducting polym