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Measurements of nitric oxide in biological materials using a porphyrinic microsensor

✍ Scribed by T. Malinski; Z. Taha; S. Grunfeld; A. Burewicz; P. Tomboulian; F. Kiechle

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
518 KB
Volume
279
Category
Article
ISSN
0003-2670

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✦ Synopsis

The application and optimization of a microsensor for in situ measurements of nitric oxide (NO) in biological systems are described. The sensor (diameter OS-O.8 pm), exhibiting a response time better than 10 ms and a detection limit of 10 nM, consists of several layers of p-type semiconducting polymeric porphyrin and cation exchanger (Nafion) deposited on a thermally-sharpened carbon fiber. The sensor has been applied to studies of NO release from a single endothelial cell in a pulmonary artery, as well as for the determination of NO in blood.

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