Measurement of nanogram quantities of UDP-glucuronic acid in tissues

✍ Scribed by Kim Ping Wong

• Book ID: 102627166
• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 267 KB
• Volume: 82
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90194-4

✦ Synopsis

A micromethod was developed for the measurement of UDP-glucuronic acid present in samples as small as 60 mg of liver, kidney, small intestine, heart, or muscle. It utilizes the highly efficient tyramine-glucuronidating system, catalyzed by LJDP-glucuronyltransferase of guinea pig liver. The reaction is specific for UDP-glucuronic acid: UDP derivatives of glucose, galactose. N-acetylglucosamine, and xylose were inactive as conjugating agents. The concentration of UDPglucuronic acid, expressed as micromoles per 100 g wet wt of the following tissues of guinea pig, rat, and mouse are, respectively: liver.