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Measurement of KLa by a gassing-in method with oxygen-enriched air

✍ Scribed by Ho Nam Chang; Benoit Halard; Murray Moo-Young

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
658 KB
Volume
34
Category
Article
ISSN
0006-3592

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✦ Synopsis

The dynamic gassing-out method using nitrogen for gas-liquid K,a measurements has been modified so that gassing-out is performed with air and gassing-in with oxygen-enriched air. This new method was proven theoretically valid for use in inert model systems and in actual fermentation systems. The K,a values were measured in a I-m-high bubble column and compared with those obtained from the traditional gassing-in method for three different mixing models in batchwise contacting: mixed gas and liquid (MMB); plug-flow gas and mixed liquid (PMB); and plug-flow gas and plug-flow liquid (PPB). The K,a values obtained f r o m the new method were consistent with those of the method of Bartholomew et al. Discrimination between the models appeared to be not important for the I-m-high column, but the theoretical analysis revealed that it would become necessary for columns about 10 m and higher.

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