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Measurement of DNA length by gel electrophoresis

โœ Scribed by E.M. Southern

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
349 KB
Volume
100
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Plotting fragment length against reciprocal of mobility gives a straight line over a wider range than the conventional semilogarithmic plot. Curvature can be removed by a simple correction. A method is also given for determining molecular weights from mobilities by direct calculation.

๐Ÿ“œ SIMILAR VOLUMES

Measurement of DNA length by gel electro
Measurement of DNA length by gel electrophoresis II: Comparison of methods for relating mobility to fragment length
โœ J.K. Elder; E.M. Southern ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 425 KB

Relationships between DNA length and electrophoretic mobility in an agarose gel have been compared by estimating the lengths of known DNA polymer fragments, using other fragments in the series as standards. Global estimates were made using 10 fragments as standards; local estimates were made using t