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Measurement of DNA breaks and oxidative damage in polymorphonuclear and mononuclear white blood cells: a novel approach using the comet assay

✍ Scribed by Lisa Giovannelli; Vanessa Pitozzi; Silvia Riolo; Piero Dolara


Book ID
117767139
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
232 KB
Volume
538
Category
Article
ISSN
1383-5718

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A modified assay for the detection of DNA single-strand breaks (SSBs) in human mononucleated white blood cells (MWBCs) based on the nick translation (NT) reaction was developed and combined with the test for unscheduled DNA synthesis (UDS). Both assays were performed on disposable 96-well filtration