The diffusion coefficient of ethanol in a 4 w/w% agarose gel at 25OC was measured, using the methods of unsteady-state diffusion into, and out of, gel beads dispersed in a solution of finite volume. The results (8.0 -9.5x10w6 cm2/s> agreed well with available theory. The results of method "out" were
โฆ LIBER โฆ
Measurement of diffusion coefficients of DNA in agarose gel
โ Scribed by Gosnell, Donna L.; Zimm, Bruno H.
- Book ID
- 120523751
- Publisher
- American Chemical Society
- Year
- 1993
- Tongue
- English
- Weight
- 651 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0024-9297
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The field free diffusion constant and the electric field dependence of the electrophoretic mobility and molecular orientation of DNA samples from 5 to 164 kilobase pairs in agarose gels from 0.5 to 2% have been measured by fluorescence recovery after photobleaching and birefringence. In conditions w