Matrix isopotential synchronous fluorescence Direct determination of gentisic acid in urine

✍ Scribed by José Antonio Murillo Pulgarín; Aurelia Alañón Molina

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 834 KB
• Volume: 296
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)85153-0

✦ Synopsis

A new fluorimetric technique is described for the determination of compounds in samples with unknown background fluorescence. The technique consists of performing synchronous scans through a trajectory which joins points of equal intensity of a fluorescent matrix tridimensional spectrum. The method is called "matrix isopotential synchronous fluorescence". This technique can be improved by application of derivatives. In order to demonstrate the validity, applicability and simplicity of the method for determining analytes in complex fluorescent matrices, it is applied to the analysis of gent& acid in urine. Better sensitivity and reproducibility are achieved in these matrices than with the fluorhnetric methods described in the literature, without the need for tedious pre-separation.