A simple, rapid, sensitive and selective method for the simultaneous determination of the non-stetoidal anti-inflammatory drug naptoxen (NAP) and its main metabolite the 6-demethylated derivative (DNAP) in human serum is described. The method is based on the intrinsic fluorescence of both acids in a
Direct Determination of Salicylic Acid in Human Serum by Matrix Isopotential Synchronous Fluorescence
β Scribed by J.A.M. Pulgarin; A.A. Molina
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 398 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0026-265X
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β¦ Synopsis
A new spectrofluorimetric method has been applied for determining salicylic acid in human serum for concentrations between 0.020 and (0.252 \mathrm{ppm}). The method was performed in an aqueous medium at (\mathrm{pH}) 6.0) attained by addition of phosphate buffer solution. The method is based on the use of a new technique called matrix isopotential synchronous fluorescence, which enables us to determine analytes in complex fluorescent matrices without the need for tedious prior separation. The sensitivity and reproducibility achieved in these matrices are better than those achieved with the fluorimetric methods described in the references. 1995 Academic Press. Inc.
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