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Direct Determination of Salicylic Acid in Human Serum by Matrix Isopotential Synchronous Fluorescence

✍ Scribed by J.A.M. Pulgarin; A.A. Molina


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
398 KB
Volume
52
Category
Article
ISSN
0026-265X

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✦ Synopsis


A new spectrofluorimetric method has been applied for determining salicylic acid in human serum for concentrations between 0.020 and (0.252 \mathrm{ppm}). The method was performed in an aqueous medium at (\mathrm{pH}) 6.0) attained by addition of phosphate buffer solution. The method is based on the use of a new technique called matrix isopotential synchronous fluorescence, which enables us to determine analytes in complex fluorescent matrices without the need for tedious prior separation. The sensitivity and reproducibility achieved in these matrices are better than those achieved with the fluorimetric methods described in the references. 1995 Academic Press. Inc.


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