Mathematical representation of electrophoretic mobility in mixed aqueous-methanolic buffers in capillary zone electrophoresis

✍ Scribed by A. Jouybana; H. K. Chan; B. J. Clark; E. Kenndler

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 81 KB
• Volume: 13
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/mcs.10017

✦ Synopsis

Abstract

In a drug development program improved speed of the development phase is constantly being explored. But much effort and time is usually expended in finding suitable methods for pharmaceutical and biomedical analyses. In contrast, a mathematical model has been presented here to correlate/predict electrophoretic mobility of acidic and basic analytes in capillary electrophoresis at different concentrations of organic modifier. This model could help analysts to find the optimum concentration of organic modifier for a successful analysis. The accuracy and predictability of the proposed model have been shown using generated mobility data (five basic drugs in water‐methanol mixed running buffer) and previously published data sets collected from a recent publication. Using this model the average percentage errors for correlative and predictive equations were 0.64 and 1.35%, respectively. The accuracy of the model was also examined against those of previously published models where the results are comparable. © 2001 John Wiley & Sons, Inc. J Micro Sep 13: 346–350, 2001