Maternal cell contamination of amniotic fluid cell cultures: Results of a U.S. nationwide survey
โ Scribed by Benn, Peter A. ;Hsu, Lillian Y. F. ;Karp, Laurence E.
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 631 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0148-7299
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Maternal cell contamination in amniotic fluid samples is easily detected by in situ hybridization if the karyotype of the fetus differs from the karyotype of the mother. One out of two amniotic fluid samples appears to contain more than 20% maternal cells. Bloody samples often contain even more than
A time proven technique is described for growing amniotic fluid cell cultures on cover glasses in Leighton tubes and for processing the mitotic cells in situ. Karyotyping the clones in situ eliminates most of the problems caused by somatic chromosome mutations in vitro and by maternal cell growth.
reason that a cytogenetic study was performed in the present case. The finding of an, apparently, non-mosaic 90,XX karyotype was surprising, given its rarity. This chromosomal constitution had to be the product of two errors: one prezygotic originating a 45,X zygote and then an endoreduplication of