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A technique forin situKaryotyping of primary amniotic fluid cell cultures

✍ Scribed by W. Schmid

Publisher
Springer
Year
1975
Tongue
English
Weight
334 KB
Volume
30
Category
Article
ISSN
0340-6717

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✦ Synopsis

A time proven technique is described for growing amniotic fluid cell cultures on cover glasses in Leighton tubes and for processing the mitotic cells in situ. Karyotyping the clones in situ eliminates most of the problems caused by somatic chromosome mutations in vitro and by maternal cell growth.

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Maternal cell contamination in amniotic
Maternal cell contamination in amniotic fluid samples as a consequence of the sampling technique
✍ Silke Nuß; Dagmar Brebaum; Caspar Grond-Ginsbach πŸ“‚ Article πŸ“… 1994 πŸ› Springer 🌐 English βš– 423 KB

Maternal cell contamination in amniotic fluid samples is easily detected by in situ hybridization if the karyotype of the fetus differs from the karyotype of the mother. One out of two amniotic fluid samples appears to contain more than 20% maternal cells. Bloody samples often contain even more than