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Mass spectrometry label-free quantitative analysis of proteins

โœ Scribed by A. T. Kopylov; V. G. Zgoda; A. I. Archakov

Book ID
111472151
Publisher
Pleiades Publishing
Year
2010
Tongue
English
Weight
221 KB
Volume
4
Category
Article
ISSN
1990-7508

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For relative protein quantitation by mass spectrometry we metabolically labeled E. coli bacteria with (15)N-enriched diets. Proteins extracted from (15)N-labeled and unlabeled E. coli bacteria were mixed, separated by two-dimensional gel electrophoresis and enzymatically digested. The resulting tryp