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Mass spectrometry-based label free quantification of gel separated proteins

✍ Scribed by Szabo, Zoltan; Szomor, Judit Szeline; Foeldi, Istvan; Janaky, Tamas


Book ID
118479125
Publisher
Elsevier
Year
2012
Tongue
English
Weight
766 KB
Volume
75
Category
Article
ISSN
1874-3919

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## Abstract In the present work we report on a novel and fast protocol for accurate bottom‐up protein quantification that overcomes the drawbacks of in‐gel digestion and MALDI analysis, while maintaining their benefits. It relies on the following steps: (i) gel electrophoresis separation of protein