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Mass spectrometry compatibility of two-dimensional gel protein stains

✍ Scribed by Wendy M. Lauber; James A. Carroll; Dawn R. Dufield; Jennifer R. Kiesel; Melissa R. Radabaugh; James P. Malone


Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
949 KB
Volume
22
Category
Article
ISSN
0173-0835

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Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry is a powerful combination for the separation of complex protein mixtures in biological samples and the subsequent identification of individual polypeptides. We have used this approach to construct a database of proteins of the