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Mass spectrometric studies of the path of carbon in photosynthesis: Positional isotopic analysis of 13C-labelled 2-octulose phosphates

✍ Scribed by Robert W. Irvine; Ian L. Flanigan; John K. MacLeod; J. Grant Collins; John F. Williams

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
767 KB
Volume
27
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

The electron impact mass spectrometric fragmentation patterns of the per‐O‐trimethylsilyl (TMS) and alkoxime‐TMS derivatives of D‐glycerol‐D‐altro and D‐glycerol‐D‐ido‐octuloses were fully analysed using seven specifically labelled ^13^C analogues. Many of the more intense‐ ions in the spectra of the per‐O‐TMS furanose and pyranose derivatives have more than one origin. On the other hand, the mass spectra of the straight‐chain methoxime and ethoxime hepta‐O‐TMS derivatives contain ions each of which in general originares from a single cleavage process. This makes gas chromatography/mass spectrometry of the aikoxime‐TMS derivatives a suitable method for monitoring ^13^C incorporations at individual carbon atoms in the corresponding octulose phosphates during photosynthesis experiments with ^13^CO~2~.

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Mass spectrometric studies of the path o
Mass spectrometric studies of the path of carbon in photosynthesis: positional isotopic analysis of 13C-labelled C4 to C7 sugar phosphates
✍ John K. MacLeod; Ian L. Flanigan; John F. Williams; J. Grant Collins 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 160 KB

## Abstract The (EIMS) electron ionization mass spectrometric fragmentation patterns of the methoxime‐ and ethoxime‐trimethylsilyl (TMS) derivatives of C~4~ to C~7~ sugars involved as phosphates in the Calvin pathway of photosynthesis in plants were analysed by gas chromatography/EIMS using specifi