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Mass spectrometric analysis of N- and O-glycosylation of tissues and cells

โœ Scribed by Stuart M Haslam; Simon J North; Anne Dell

Book ID
113883113
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
497 KB
Volume
16
Category
Article
ISSN
0959-440X

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In-gel digestion of densely O-glycosylated proteins, an essential step in proteome analysis, is often hampered by steric hindrance of the proteases. To overcome this technical problem a simple and convenient method has been developed, which combines several advantages: (1) Approximately 70% of the o