Mass Cytometry: Methods and Protocols

✍ Scribed by Helen M. McGuire, Thomas M. Ashhurst

• Publisher: Springer New York; Humana
• Year: 2019
• Tongue: English
• Leaves: 333
• Series: Methods in Molecular Biology 1989
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This book details up-to-date and cutting edge compilation of protocols in mass cytometry. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mass Cytometry: Methods and Protocols aims to ensure successful results in the further study of this vital field.

✦ Table of Contents

Front Matter ....Pages i-xii
Front Matter ....Pages 1-1
Setting Up Mass Cytometry in a Shared Resource Lab Environment (Sarah Warth, Désirée Kunkel)....Pages 3-11
Acquisition, Processing, and Quality Control of Mass Cytometry Data (Brian H. Lee, Adeeb H. Rahman)....Pages 13-31
Front Matter ....Pages 33-33
Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold Gating (Amelia Au-Yeung, Chikara Takahashi, W. Rodney Mathews, William E. O’Gorman)....Pages 35-45
Method for Tagging Antibodies with Metals for Mass Cytometry Experiments (Stephen Gregory Chang, Cynthia J. Guidos)....Pages 47-54
Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for Single-Cell Mass Cytometry Analysis (Felix J. Hartmann, Erin F. Simonds, Nora Vivanco, Trevor Bruce, Luciene Borges, Garry P. Nolan et al.)....Pages 55-81
Titration of Mass Cytometry Reagents (Caryn van Vreden, Paula Niewold, Helen M. McGuire, Barbara Fazekas de St. Groth)....Pages 83-92
Surface Barcoding of Live PBMC for Multiplexed Mass Cytometry (Axel Ronald Schulz, Henrik E. Mei)....Pages 93-108
Front Matter ....Pages 109-109
Automated Cell Processing for Mass Cytometry Experiments (Jaromir Mikes, Axel Olin, Tadepally Lakshmikanth, Yang Chen, Petter Brodin)....Pages 111-123
Live Cell Barcoding for Efficient Analysis of Small Samples by Mass Cytometry (Lisa E. Wagar)....Pages 125-135
Front Matter ....Pages 137-137
Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry (Diana Shinko, Thomas M. Ashhurst, Helen M. McGuire, Kellie A. Charles)....Pages 139-146
Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometry (Yannick Simoni, Michael Fehlings, Evan W. Newell)....Pages 147-158
Analysis of the Murine Bone Marrow Hematopoietic System Using Mass and Flow Cytometry (Thomas M. Ashhurst, Darren A. Cox, Adrian L. Smith, Nicholas J. C. King)....Pages 159-192
Mass Cytometric Cell Cycle Analysis (Gregory K. Behbehani)....Pages 193-215
Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry (Mikael Roussel, Todd Bartkowiak, Jonathan M. Irish)....Pages 217-226
Quantitative Measurement of Cell-Nanoparticle Interactions Using Mass Cytometry (Andrew J. Mitchell, Angela Ivask, Yi Ju)....Pages 227-241
Front Matter ....Pages 243-243
Data-Driven Flow Cytometry Analysis (Sherrie Wang, Ryan R. Brinkman)....Pages 245-265
Analysis of Mass Cytometry Data (Christina B. Pedersen, Lars R. Olsen)....Pages 267-279
Analysis of High-Dimensional Phenotype Data Generated by Mass Cytometry or High-Dimensional Flow Cytometry (Branko Cirovic, Natalie Katzmarski, Andreas Schlitzer)....Pages 281-294
Computational Analysis of High-Dimensional Mass Cytometry Data from Clinical Tissue Samples (Sam Norton, Roslyn Kemp)....Pages 295-307
Supervised Machine Learning with CITRUS for Single Cell Biomarker Discovery (Hannah G. Polikowsky, Katherine A. Drake)....Pages 309-332
Back Matter ....Pages 333-338

✦ Subjects

Life Sciences; Cell Biology; Mass Spectrometry; Antibodies