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Mapping the chromosome instability function of the SV40 T antigen

✍ Scribed by F.Andrew Ray; Mary Jo Waltman; Paul M. Kraemer


Book ID
119106021
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
118 KB
Volume
66
Category
Article
ISSN
0165-4608

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## Abstract The expression of the nuclear SV40‐induced T antigen was measured by micro fluorimetry on individual, asynchronously growing SV40‐transformed cells which had been stained with hamster T‐antiserum by the indirect immunofluorescence method. The same individual cells were first measured fo

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The SV40 T antigen causes numerical (aneuploidy) and structural (aberrations) chromosome damage when expressed in human diploid fibroblasts. This chromosome damage precedes the acquisition of neoplastic traits such as anchorage independence, colony formation in reduced serum growth factors, immortal