Mapping protein–protein interactions with combinatorial biology methods

✍ Scribed by Joelle Pelletier; Sachdev Sidhu

• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 95 KB
• Volume: 12
• Category: Article
• ISSN: 0958-1669
• DOI: 10.1016/s0958-1669(00)00225-1

✦ Synopsis

Extremely diverse, DNA-encoded libraries of peptides and proteins have been constructed that include a linkage between each polypeptide and the encoding DNA. Library members can be selected by virtue of a particular binding specificity, and their protein sequence can be deduced from the sequence of the cognate DNA. Such combinatorial biology methods have proven invaluable in both identifying natural protein-protein interactions and also in mapping the specificities and energetics of these interactions in fine detail.