New molecular biology methods for protein engineering

✍ Scribed by Mark J. Zoller

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 700 KB
• Volume: 2
• Category: Article
• ISSN: 0958-1669
• DOI: 10.1016/0958-1669(91)90076-h

✦ Synopsis

This review outlines recent advances in the application of molecular biological techniques to the study of protein structure and function. The chapter is divided into four main sections: methods for oligonucleotide-directed mutagenesis; mutational strategies for identifying functional residues and domains; systems for expression; and future developments. Few new methods were reported in 1990; however, a number of the papers that appeared represent refinements of previously reported strategies. This review is also published in Current Opinion in Structural Biology 1991, 1:605-610.