Mapping of the natural resistance-associated macrophage protein 1 (NRAMP1) gene to pig chromosome 15

✍ Scribed by H. S. Sun; L. Wang; M. F. Rothschild; C. K. Tuggle

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 116 KB
• Volume: 29
• Category: Article
• ISSN: 0268-9146
• DOI: 10.1046/j.1365-2052.1998.00298.x

✦ Synopsis

The natural resistance‐associated macrophage protein 1 (NRAMP1) was mapped in the pig for study as a potential candidate gene in controlling pig resistance to Salmonella infection. Primers were designed from the pig cDNA to amplify a 1·6 kb fragment between exons 1 and 3. By using a pig‐rodent somatic cell hybrid panel, NRAMP1 was mapped to pig chromosome 15 (SSC15) with 100% probability, and the regional assignment was SSC15q23‐26 with 87% concordance. A polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) marker was developed by using the __Hinf__I enzyme and three alleles were identified from a population including 11 breeds. Linkage analysis confirmed the physical assignment by using the PiGMaP reference families. Pig NRAMP1 was linked to SSC15 markers S0088, S0149 and S0284 (LOD > 3). A small population study revealed large allele frequency differences among tested breeds. An A allele is only observed in dam (white) lines whereas a similar exclusivity of the C allele was seen in sire (colored) breeds.