Genes specifying the oxidative utilization of hydroxyproline (Hyp) in P. aeruginosa PAO were located on the chromosome, around 19th minute by conjugation experiments. A map order of his-68-his-07-Hyp was assigned. Confirmation of this gene order was also demonstrated by transductional mapping studie
Mapping of the locus involved in the catabolic oxidation of D-amino acids in Pseudomonas aeruginosa PAO
โ Scribed by Manoharan, T. Herbert ;Jayaraman, Kunthala
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 533 KB
- Volume
- 164
- Category
- Article
- ISSN
- 0026-8925
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โฆ Synopsis
Mutants of Pseudomonas aeruginosa PAO deficient in their utilization of DL-valine have been found to have lost their capacity to utilize DL-alanine and L-proline. Use of conjugal and transductional mediated gene transfers have established the chromosomal location of this gene and also its pleotropic function in the induction of the D-amino acid oxidase, involved in the oxidative utilization of DL-valine, DL-alanine and L-proline. These point mutations are clustered in a single locus designated as Val D and mapped around the 19th minute on the chromosome.
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