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Mapping of mitochondrial DNA fromTorulopsis glabrata

✍ Scribed by G. D. Clark-Walker; K. S. Sriprakash; C. R. McArthur; A. A. Azad

Publisher
Springer-Verlag
Year
1980
Tongue
English
Weight
885 KB
Volume
1
Category
Article
ISSN
0172-8083

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✦ Synopsis

Large and small rRNAs have been isolated from mitochondria of the yeast Torulopsis glabrata and have been shown to have lengths of 2,700 bases and 1,400 bases respectively. Construction of a restriction endonuclease site map of mitochondrial DNA has enabled us to position these rRNAs by hybridization of labelled RNA to DNA fragments transferred to nitrocellulose. The large and small mt rRNA genes are separated by a minimum of 1,820 bp and a maximum of 2,765 bp on the 18,870 bp mitochondrifil genome. tRNA genes map within this separating sequence but they are also located distal to both rRNA genes. The implication of these results to the structural relationships of mitochondrial DNAs from yeasts is discussed.

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