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Genetic map of mitochondrial DNA inPodospora anserina

✍ Scribed by Ulrich Kück; Karl Esser

Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
521 KB
Volume
5
Category
Article
ISSN
0172-8083

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✦ Synopsis

In order to develop an eukaryotic vector with the Podospora plasmid, further characterization is required of the mitochondrial DNA into which this plasmid is integrated, a physical map (restriction sites) of the Podospora chondriome (size 95 kb) has been completed. As prerequisite for the establishment of a genetic (functional) map, 70% of the chondriome was cloned in E. eoli vectors. Using mitochondrial genes from Saccharomyces cerevisiae, six structural genes were located on the Podospora chondriome by cross hybridization experiments. There is strong evidence that the plasmid is inserted into the cytochrome b gene. A comparison of the genetic map of the Podospora chondriome with those of Neurospora crassa and Aspergillus nMulans exhibits a rather good accordance with respect to the sequence of genes.

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