Mapping and expression of a bifunctional thymidylate synthase, dihydrofolate reductase gene from maize
β Scribed by Kevin Cox; Dominique Robertson; Roger Fites
- Book ID
- 110234246
- Publisher
- Springer
- Year
- 1999
- Tongue
- English
- Weight
- 135 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0167-4412
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π SIMILAR VOLUMES
The bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) of Daucus carom has been further characterized as regards molecular weight, amino acid composition, protease digestion and microsequencing of proteolytic peptides. Data reported in this paper demonstrate that the carrot protein
## Background Dihydrofolate reductase (DHFR) and thymidylate synthase (TS) are crucial enzymes in DNA synthesis. In alveolata both enzymes are expressed as one bifunctional enzyme. ## Results Loss of this essential enzyme activities after successful allelic assortment of knock out alleles yields