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Mapping and expression of a bifunctional thymidylate synthase, dihydrofolate reductase gene from maize

✍ Scribed by Kevin Cox; Dominique Robertson; Roger Fites


Book ID
110234246
Publisher
Springer
Year
1999
Tongue
English
Weight
135 KB
Volume
41
Category
Article
ISSN
0167-4412

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## Background Dihydrofolate reductase (DHFR) and thymidylate synthase (TS) are crucial enzymes in DNA synthesis. In alveolata both enzymes are expressed as one bifunctional enzyme. ## Results Loss of this essential enzyme activities after successful allelic assortment of knock out alleles yields