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Biochemical Evidence for the Presence of a Bifunctional Dihydrofolate Reductase-Thymidylate Synthase in Plant Species

✍ Scribed by A. Balestrazzi; M. Branzoni; D. Carbonera; B. Parisi; R. Cella


Book ID
117391548
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
619 KB
Volume
147
Category
Article
ISSN
0176-1617

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Molecular cloning of dihydrofolate reductase-thymidylate synthase (DHFR-TS) of Daucus carom was achieved by immunoscreening of a cDNA library obtaining a 2 kbp clone which contains an open reading frame of 1528 bp. Comparison of the deduced amino acid sequence with those from other sources revealed