Hemoglobin has been previously shown to unfold during freeze drying when lyophilized from formulations that undergo freeze-concentration induced phase separation (Heller et al. 1997. Biotechnol Prog 13:590-596). In this report, we show that such damage may be avoided using kinetic strategies to arre
โฆ LIBER โฆ
Manipulation of Lyophilization-Induced Phase Separation: Implications For Pharmaceutical Proteins
โ Scribed by Martin C. Heller; John F. Carpenter; Theodore W. Randolph
- Book ID
- 109388017
- Publisher
- American Institute of Chemical Engineers
- Year
- 1997
- Tongue
- English
- Weight
- 211 KB
- Volume
- 13
- Category
- Article
- ISSN
- 8756-7938
No coin nor oath required. For personal study only.
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A water-soluble polymer such as polyethylene glycol (PEG), Dextran T-500 (Dx), or diethylaminoethyl-Dextran (DEAE-Dx) induced aqueous micellar solutions of octyl-โค-D-thioglucoside (OTG) to phase separation at 0ยฐC. One of the two phases thus formed is a surfactantdepleted aqueous solution (aqueous ph