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Manganese-guided cellular MRI of human embryonic stem cell and human bone marrow stromal cell viability

✍ Scribed by Mayumi Yamada; Paul T. Gurney; Jaehoon Chung; Pratima Kundu; Micha Drukker; Alan K. Smith; Irving L. Weissman; Dwight Nishimura; Robert C. Robbins; Phillip C. Yang

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
582 KB
Volume
62
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

This study investigated the ability of MnCl~2~ as a cellular MRI contrast agent to determine the in vitro viability of human embryonic stem cells (hESC) and human bone marrow stromal cells (hBMSC). Basic MRI parameters including T~1~ and T~2~ values of MnCl~2~‐labeled hESC and hBMSC were measured and viability signal of manganese (Mn^2+^)‐labeled cells was validated. Furthermore, the biological activity of Ca^2+^‐channels was modulated utilizing both Ca^2+^‐channel agonist and antagonist to evaluate concomitant signal changes. Metabolic effects of MnCl~2~‐labeling were also assessed using assays for cell viability, proliferation, and apoptosis. Finally, in vivo Mn^2+^‐guided MRI of the transplanted hESC was successfully achieved and validated by bioluminescence imaging. Magn Reson Med, 2009. © 2009 Wiley‐Liss, Inc.

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