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Mammalian cell delivery via aerosol deposition

✍ Scribed by William. S. Veazey; Kenneth J. Anusavice; Karen Moore

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
155 KB
Volume
72B
Category
Article
ISSN
1552-4973

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✦ Synopsis

Abstract

The objective of this study was to test the hypothesis that bovine dermal fibroblasts can survive aerosol delivery via an airbrush with mean cell survival rates greater than 50%. This technology has great implications for burn and other wound therapies, for delivery of genetically altered cells in gene therapies, and for tissue engineering with tissue scaffolds. Bovine dermal fibroblasts were suspended at a concentration of 200,000 cells/mL in Hank's Balanced Salt Solution, and delivered into six‐well tissue culture plates using a Badger 100G airbrush. Cells were delivered through three nozzle diameters (312, 484, and 746 ΞΌm) at five different air pressures (41, 55, 69, 96, and 124 kPa). Nine repetitions were performed for each treatment group, and cell viability was measured using trypan blue exclusion assay. Mean cell viability ranged from 37 to 94%, and depended on the combination of nozzle diameter and delivery pressure (p < 0.0001). Linear regression analysis was used to develop a stochastic model of cell delivery viability as a function of nozzle diameter and delivery air pressure. This study demonstrates the feasibility of using an airbrush to deliver viable cells in an aerosol to a substrate. Β© 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 72B: 334–338, 2005

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