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✦   LIBER   ✦

Aerosol delivery of mammalian cells for tissue engineering

✍ Scribed by Andrew Roberts; Barbara E. Wyslouzil; Lawrence Bonassar

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
252 KB
Volume
91
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

We developed a two fluid atomizer to spray mammalian cells suspended in phosphate buffered saline (PBS)–Pluronic F‐127 polymer solutions. We used the device to spray suspensions of bovine articular chondrocytes and porcine tracheal epithelial cells, in either PBS or the polymer solutions, and studied their viability and growth kinetics. Under the operating conditions examined in this study, the cells had better than 70% viability after spraying, the growth rates were comparable to cells that were not sprayed, and the appearance of the cells at confluence was normal. Β© 2005 Wiley Periodicals, Inc.

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