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Malignant transformation in vitro of mouse fibroblasts by 7, 12-dimethylbenz(A)anthracene and 7-hydroxymethylbenz(A)anthracene and by their K-region derivatives

✍ Scribed by H. Marquardt; J. E. Sodergren; P. Sims; P. L. Grover

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
420 KB
Volume
13
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

K‐region epoxides derived from 7, 12‐dimethylbenz(a)anthracene and from 7‐hydroxymethlybenz(a)anthracene were tested for their ability to induce malignant transformation of the M2 clone of fibroblasts derived from C3H mouse prostate. The parent hydrocarbons, the corresponding K‐region dihydrodiols and a phenol were also tested with these cells. The K‐region epoxides induced transformation but were somewhat less active than the hydrocarbons; the ring‐hydroxylated derivatives were inactive. In other experiments, the addition of α‐naphthoflavone was found to inhibit the formation of water‐soluble metabolites from and the toxicity of 7, 12‐dimethylbenz(a)anthracene without affecting malignant transformation. The results are discussed in relation to current theories regarding the metabolic activation of polycyclic hydrocarbons.

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