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Malignant Melanoma in Children and Congenital Melanocytic Nevi: DNA Content and Cell Cycle Analysis by Flow Cytometry

✍ Scribed by Antonio Alvarez-Mendoza; Jorge Reyes-Esparza; Ramon Ruiz-Maldonado; Eduardo Lopez-Corella; Norma C. Juarez-Herrera


Publisher
Springer
Year
2001
Tongue
English
Weight
719 KB
Volume
4
Category
Article
ISSN
1093-5266

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Background: Analysis of the DNA cell cycle and glutathione content cannot be performed on viable cells, because the fluorescence emissions of the DNA-specific probe Hoechst 33342 and the glutathione-specific probe monobromobimane overlap completely. We decided to explore whether the emissions could