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Simultaneous analysis of mitochondrial activity and DNA content in ehrlich ascites tumor cells by dual parameter flow cytometry

✍ Scribed by T. Hämmerle; M. Löffler

Publisher
Springer
Year
1989
Tongue
English
Weight
622 KB
Volume
93
Category
Article
ISSN
1432-119X

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✍ Jan F. Keij; Carolyn Bell-Prince; John A. Steinkamp 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 106 KB 👁 2 views

Background: Analysis of the DNA cell cycle and glutathione content cannot be performed on viable cells, because the fluorescence emissions of the DNA-specific probe Hoechst 33342 and the glutathione-specific probe monobromobimane overlap completely. We decided to explore whether the emissions could