The cross-reactivity of several monoclonal antibodies recognizing monomorphic determinants of human HLA-A, B, C, and DR antigens and human beta 2-microglobulin (beta 2m) has been studied on peripheral blood leukocytes in 24 different species. An monoclonal HLA-A-, B-, and C-specific antibody and fou
Major Histocompatibility Gene Therapy: The Importance of Haplotype and β2-Microglobulin
✍ Scribed by Frank N. Salamone; Lyon L. Gleich; Ya-Qin Li; Peter J. Stambrook
- Book ID
- 110085671
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 101 KB
- Volume
- 114
- Category
- Article
- ISSN
- 0023-852X
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DNA of unrelated persons as well as members of families that were totally or partially homozygous or completely heterozygous on the loci of the major histocompatibility class I genes has been isolated from peripheral blood lymphocytes and blot hybridized with the class I pseudogene pHLA 12.4 probe.
With P,-microglobulin-( P2m-) cell lines such as RIE/Db, the surface expression of class I major histocompatibility complex molecules is greatly impaired, and class I molecules that are on the surface are generally misfolded. To determine whether P2m must be continually present with the class I heav