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Maintaining perpetual synchrony in HeLa S3 culture

✍ Scribed by W. G. Thilly; T. S. Nowak Jr.; G. N. Wogan


Book ID
101723252
Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
394 KB
Volume
16
Category
Article
ISSN
0006-3592

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Unbalanced growth and cell death in HeLa
✍ Oskar S. Frankfurt πŸ“‚ Article πŸ“… 1981 πŸ› John Wiley and Sons 🌐 English βš– 616 KB

## Abstract Flow cytometry indicated that significant amounts of dsRNA were accumulated in HeLa S3 cells blocked at or near G~1~/S boundary by hydroxyurea (HU) or excess thymidine (TdR). The dsRNA/DNA ratio increased in these cells in a manner characteristic of unbalanced cell growth. In HU‐treated