In D. melanogaster, many ribosomal genes contain an intervening sequence that interrupts the 28S rRNA gene. In wild-type flies, type I and type II insertions are rarely transcribed and the transcripts of the interrupted genetic units do not contribute significantly to the production of mature riboso
Magnification of the rDNA cluster in Kluyveromyces lactis
โ Scribed by Maleszka, R. ;Clark-Walker, G. D.
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 373 KB
- Volume
- 223
- Category
- Article
- ISSN
- 0026-8925
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โฆ Synopsis
By employing pulsed field gel electrophoresis we find that slow growing strains of Kluyveromyces lactis have only 43%-55% of the wild-type level of ribosomal DNA (rDNA) repeats. When subjected to prolonged vegetative growth these strains can increase both the number of rDNA repeats and their growth rate.
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The inductive effect of lactose,S-methyl-thio-g-galactopyranoside, (TMG) and glucose on B galactosidase synthesis in Kluyveromyces lactis has been \_ .~ ---\_~ studied. Whereas TMG gave a five fold stimulation of the rate of @galactosidase synthesis, lactose only gave a small stimulation. Glucose c
D. melanogaster males of XYbb/O genetic constitution undergoing rDNA magnification were mated singly to XXbb+/O females, yielding XYbb/O male progeny, and to XNO- w sn bb+ females, yielding XYbb/XNO- females. The male and female offspring were scored for the bb+ phenotype. Results show that there is