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Lysogenic induction of lambdoid phages in lexA mutants of Escherichia coli

✍ Scribed by Sedgwick, Steven G. ;Yarranton, Geoffrey T. ;Heath, Roy W.

Publisher: Springer
Year: 1981
Tongue: English
Weight: 293 KB
Volume: 184
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00352522

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✦ Synopsis

UV irradiation of lexA3 mutants of E. coli caused lysogenic induction of prophage lambda, lambda i21, lambda i434 and phi 80. Maximal induction in lexA3 lysogens needed less UV than in lexA+ bacteria and gave 25-100% of the normal levels of infective centres induced. Assays of gene expression arising from derepression of a defective lambda prophage showed at least 40% of the normal levels of induction by mitomycin C in lexA3 bacteria. The need for post-irradiation protein synthesis for lysogenic induction in lexA3 lysogens was reduced by increasing the basal level of recA protein with a recA+ plasmid. It is concluded that in lexA E. coli some recA protein synthesis, too small to be detected by physical means, is needed for UV induced lysogenic induction.

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