✦ LIBER ✦

A method for the isolation of phage mutants altered in their response to lysogenic induction

✍ Scribed by Mount, David W.

Publisher: Springer
Year: 1976
Tongue: English
Weight: 286 KB
Volume: 145
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00269589

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✦ Synopsis

Phage lambdacl+ gives clear plaques whereas phage lambdacIind- gives turbid plaques on a lawn of a mutant strain of E. coli K12. This strain, called STS, carries mutation spr in a tif sfi genetic background. I hypothesize that upon temperate phage infection, STS bacteria spontaneously inactivate phage repressor by the same mechanism involved in normal lysogenic induction which results in obligatory lytic growth of lambda+. The use of the STS mutant facilitates the isolation and genetic analysis of phage mutants with an abnormal response to lysogenic induction.

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