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Lysis ofMicrococcus lysodeikticuscells by lysozyme covalently immobilized on the lumen of hollow fibers

✍ Scribed by Cheng-Kang Lee; Mei-Chun Ku

Book ID: 104649615
Publisher: Springer-Verlag
Year: 1994
Tongue: English
Weight: 459 KB
Volume: 8
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00161589

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✦ Synopsis

A lytic enzyme reactor for microbial cell lysis is described in which lysozyme is immobilized on the lumen of hemodialyzer hollow fibers using epichlorohydrin as a coupling agent. The cell suspension flows through the lumen without any hindrance where the cells are lyzed by the immobilized lysozyme efficiently. Micrococcus lysodeikiicus cells at concentrations of 0.25 g/L and 5 g/L were successfirlly lyzed without clogging the hollow fiber. In comparison with lysozyme immobilized on submicron particles, the activity retention was at least 8 times higher.

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