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Lyotropic salt effects in hydrophobic chromatography

✍ Scribed by A. Hirotoshi Nishikawa; Pascal Bailon

Book ID
102628127
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
348 KB
Volume
68
Category
Article
ISSN
0003-2697

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✦ Synopsis

Pure hydrophobic chromatography can be observed with agarose gels containing .caprylyl/~ydrazide. These nonionic gels show increased avidity in protein adsorption with ~higher content of caprylyl groups. Lyotropic salt effects can :be used to control .chromatographic behavior of proteins. Salting-out agents enhance binding of protein :to sorbent, whi~le salting-in agents diminish this binding. ~Based on 'fhese ;findings, ~he .hydrophobic separation of/34actog10bulin, ovalbumin, and bovine serum albumin is demonstrated.

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